|Flow Cytometry (Flow)||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunohistochemistry (Paraffin) (IHC (P))||See 32 publications below|
|Western Blot (WB)||See 6 publications below|
|Miscellaneous PubMed (MISC)||See 3 publications below|
|Immunohistochemistry (IHC)||See 506 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 9 publications below|
|Immunocytochemistry (ICC)||See 43 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Dog , Rabbit , Rat , Pig , Sheep , Bovine , Mouse , Human , Rhesus monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the human Ki-67 protein|
|Storage buffer||tissue culture supernatant|
|Contains||15mM sodium azide|
|Storage conditions||4° C|
MA5-14520 targets Ki-67 in Immunohistochemistry (P) applications and shows reactivity with Human samples. Staining of formalin-fixed tissues requires boiling tissue section in 10 mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at room temperature for 20 minutes. Suggested positive controls include spleen, tonsil or breast carcinomas.
The MA5-14520 immunogen is a synthetic peptide derived from the human Ki-67 protein.
Ki-67 is a nuclear protein, which is expressed in proliferating cells. Ki-67 is preferentially expressed during late G1-, S-, M-, and G2-phases of the cell cycle, while cells in the G0 (quiescent) phase are negative for this protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Antigen identified by monoclonal antibody Ki-67; Antigen KI-67; Proliferation marker protein Ki-67; proliferation-related Ki-67 antigen; protein phosphatase 1, regulatory subunit 105
Gene Aliases: KIA; MIB-; MIB-1; MKI67; PPP1R105
UniProt ID: (Human) P46013
Entrez Gene ID: (Human) 4288
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