|Flow Cytometry (Flow)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||0.5-1.0 µg/ml|
|Immunocytochemistry (ICC)||See 5 publications below|
|Gel Shift (GS)||See 4 publications below|
|Western Blot (WB)||See 21 publications below|
|Immunoprecipitation (IP)||See 3 publications below|
|Blocking Assay (BLOCK)||See 1 publications below|
|Tested Species reactivity||Human, Non-human primate|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human B cell nuclei from plasmacytoid 2p68 cells|
|Purification||Ammonium sulfate precipitation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-12930 targets Ku (p80) in FACS, IF, IP, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-12930 immunogen is human B cell nuclei from plasmacytoid 2p68 cells.
The Ku autoantigen is a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins. The p70/p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 80kD); 80kDa; 86 kDa subunit of Ku antigen; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; complementing defective; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; Ku autoantigen; Ku autoantigen, 80kDa; Ku80; Ku86; Ku86 autoantigen related protein 1; lupus; Lupus Ku autoantigen protein p86; Nuclear factor IV; repair in Chinese hamster; Thyroid-lupus autoantigen; TLAA; X-ray repair; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining; X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5
Gene Aliases: G22P2; KARP-1; KARP1; KU80; Ku86; KUB2; NFIV; XRCC5
UniProt ID: (Human) P13010
Entrez Gene ID: (Human) 7520
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