|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:100 - 1:1000|
|Immunocytochemistry (ICC)||See 5 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 2 publications below|
|Western Blot (WB)||See 5 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rabbit , Rat , Human Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C G(400) L K R H H T G Y E Q F(411)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-655 detects lysosome-associated membrane protein-2 (LAMP2) from rat, mouse and human tissues.
PA1-655 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry. By Western blot, this antibody detects glycosylated and non-glycosylated LAMP-2, depending on the lysis buffer used.
PA1-655 immunizing peptide corresponds to amino acid residues 400-411 from rat LAMP2. This sequence is completely conserved between rat, mouse, and guinea pig LAMP2. PA1-655 immunizing peptide (Cat. # PEP-039) is available for use in neutralization and control experiments.
Lysosomes are membrane-bound vacuoles which play a critical role in cellular metabolism. These organelles participate in endocytosis, the synthesis and assembly of acid hydrolases, act as sites for digestion of foreign materials and for specialized autolytic cellular processes. Membrane-associated glycoproteins have an important role in normal lysosomal function. Prior to posttranslational modification, Lysosome-associated membrane protein-2 (LAMP-2) is an ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides with a total molecular weight of ~100-110 kDa. LAMP2 has a structure comprised of a large amino-terminal intra-lysosomal domain, a hydrophobic trans-membrane domain, and a short carboxyl-terminal cytoplasmic tail.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD107 antigen-like family member B; CD107b; LAMP-2; LAMPB; LGP-110; LGP-96; LGP-B; LGP110; lysosomal membrane glycoprotein 2; Lysosomal membrane glycoprotein type B; lysosomal-associated membrane protein 2; Lysosome-associated membrane glycoprotein 2; lysosome-associated membrane protein 2
Gene Aliases: CD107b; Lamp II; LAMP-2; Lamp-2a; Lamp-2b; Lamp-2c; LAMP2; LAMPB; LGP-B; LGP110; Mac3
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