|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:200|
|Western Blot (WB)||See 1 publications below|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Pore complex-lamina fraction isolated from rat liver nuclear envelopes.|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-074 detects lamin associated polypeptide (LAP) 1A, 1B and 1C from rat, human, and mouse samples. This antibody does not cross-react with LAP 2.
MA1-074 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects 3 distinct proteins of molecular weights ~75, 68 and 55 kDa representing LAP 1A, 1B and 1C, respectively in rat liver nuclear envelope fractions. Immunofluorescence staining of LAP 1 in rat liver with MA1-074 results in exclusive labeling of the nuclear periphery and exhibits co-localization with lamin staining.
The MA1-074 immunogen is pore complex-lamina fraction isolated from rat liver nuclear envelopes.
In eukaryotic cells, the nuclear envelope consists of inner and outer membranes which define the border between the nucleus and cytoplasm. The inner and outer nuclear membranes are joined by large, supramolecular assemblies called nuclear pore complexes through which various molecules pass into and out of the nucleus. The inner membrane is lined by a filamentous meshwork called the nuclear lamina made up of proteins called lamins.
The attachment of the lamins to the inner nuclear membrane appears to occur through their association with 4 related integral membrane proteins called Lamin Associated Polypeptides (LAPs). LAP 1A, 1B and 1C share a high degree of sequence homology while LAP 2 is distinct from the others. LAP 1A and B bind lamins A, C and B1, while LAP 2 binds lamin B1 only. In addition to lamin binding, LAP 2 has been shown to bind to chromosomes. Phosphorylation of LAP 2 during mitosis inhibits binding to both lamins and chromosomes suggesting that LAP 2 plays an important role in nuclear envelope re-assembly.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Lamin Associated Polypeptides; Lamin-associated protein 1B; Lamina-associated polypeptide 1B; Lamina-associated polypeptide 1C; LAP1B; LAP1C; torsin A interacting protein 1; Torsin-1A-interacting protein 1
Gene Aliases: LAP1; LAP1B; Lap1c; TOR1AIP1
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