|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of protein|
|Western Blot (WB)||0.5-2 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Tested Species reactivity||Bacteria, Bovine, Dog, Hamster, Human, Mouse, Non-human primate, Pig, Rat, Zebrafish|
|Published species reactivity||Rat , Mouse , Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide between amino acids 1-100 of the human LC3 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
The reaction with mouse LC3 is weaker than human.
The immunogen for this antibody production has 100% sequence homology with rat and zebrafish, 92% with mouse, and 91% with bovine proteins.
LC3, a mammalian homologue of Apg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. However, LC3 is now thought to also be involved in autophagy. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. LC3-II is the first mammalian protein identified that specifically associates with the autophagosome membranes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; Lc3; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 2 a; MAP1A/1B light chain 3 B; MAP1A/b light chain 3 B antibody; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3B; Microtubule-associated proteins 1A/1B light chain 3B MAP1A/MAP1B LC3 B antibody
Gene Aliases: 1010001C15Rik; Atg8; ATG8F; BOS_17038; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1LC3; MAP1LC3B; MAP1LC3B-a; Mpl3; wu:fb60g11; zbs559; zgc:56434
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