|Flow Cytometry (Flow)||Assay-Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:400|
|Immunoprecipitation (IP)||20 µg/500 µg of lysate|
|Western Blot (WB)||2 µg/ml|
|Western Blot (WB)||See 6 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Rat, Zebrafish|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region between residues 25-121 of human LC3 protein.|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||-20°C or -80°C if preferred|
This antibody detects both LC3A and LC3B.
In Western blot bands are seen ~17 and 19 kDa.
Suggested positive control: human and mouse brain lysate, antigen standard for MAP1LC3B (transient overexpression lysate), mouse brain protein, human brain protein.
Autophagy is a process of intracellular bulk degradation in which cytoplasmic components, including organelles, are sequestered within double-membrane vesicles that deliver the contents to the lysosome/vacuole for degradation.
During macroautophagy, the sequestering vesicles, termed autophagosomes, fuse with the lysosome or vacuole resulting in the delivery of an inner vesicle (autophagic body) into the lumen of the degradative compartment.
There are 16 proteins participating in the autophagy pathway in human. The autophagy protein LC3, a mammalian homologue of Atg8, was originally identified as microtubule-associated protein 1 light chain 3. It is a component of both the MAP1A and MAP1B microtubule-binding domains and the heavy-chain independent regulation of LC3 expression might modify MAP1 microtubule-binding activity during development. LC3 is the only known mammalian protein identified that stably associate with the autophagosome membranes. LC3-I is cytosolic and LC3-II is membrane bound and enriched in the autophagic vacuole fraction. The detection of LC3-I to LC3-II conversion is a useful and sensitive marker for distinguishing autophagy in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Autophagy-related protein LC3 A; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 A; Autophagy-related ubiquitin-like modifier LC3 B; LC3; MAP1 light chain 3-like protein 1; MAP1 light chain 3-like protein 2; MAP1A/1B light chain 3 A; MAP1A/1B light chain 3 B; MAP1A/MAP1B LC3; MAP1A/MAP1B LC3 A; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 A; MAP1A/MAP1B light chain 3 B; MAP1LC3; MAP1LC3A; MAP1LC3B; Microtubule-associated protein 1 light chain 3 alpha; Microtubule-associated protein 1 light chain 3 beta; microtubule-associated protein 1-light chain 3A; Microtubule-associated proteins 1A/1B light chain; microtubule-associated proteins 1A/1B light chain 3; Microtubule-associated proteins 1A/1B light chain 3A; Microtubule-associated proteins 1A/1B light chain 3B
Gene Aliases: 1010001C15Rik; 1010001H21Rik; 4922501H04Rik; Atg8; ATG8E; ATG8F; LC3; LC3A; LC3B; MAP1A/1BLC3; MAP1ALC3; MAP1BLC3; Map1lc3; MAP1LC3A; MAP1LC3B; MAP1LC3B-a; Mpl3; zbs559; zgc:77094
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.