|ELISA (ELISA)||Assay Dependent|
|Western Blot (WB)||0.1 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Recombinant human protein purified from E.coli (His-LDHA)|
|Storage buffer||HEPES with 0.15M NaCl, 0.01% BSA, 50% glycerol|
|Contains||0.03% sodium azide|
A suggested positive control for this product is HeLa cells.
Lactate dehydrogenase (LDH) is the enzyme in the glycolytic pathway that converts pyruvate to lactate with concomitant interconversion of NADH and NAD+. In mammals, the enzyme is encoded by three genes: LDHA (M or muscle form), LDHB (H or heart form), and LDHC (X or testis form). The three human LDHs have 84-89% sequence similarities and 69-75% amino acid identities. There are five different LDH isoenzymes based on the proportion of M and H chains existing in the LDH tetrameric structure. The LDHA is hypoxia inducible and its expression is directly controlled by the transcriptional activity of the hypoxia inducible factor 1a (HIF1a). LDHB is also known to be upregulated in many cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; L-lactate dehydrogenase A chain; lactate dehydrogenase 1, A chain; lactate dehydrogenase A-like; lactate dehydrogenase A4; lactate dehydrogenase M; lactate dehydrogenase-A; LDH muscle subunit; LDH-A; LDH-M; LDH1; LDHM; M-LDH; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; LDHA; LDHM; PIG19
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.