|Western Blot (WB)||1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide ETYRRGESGLPAHPEVPD, corresponding to amino acids 630-647 of Human LIM Kinase.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-31026 detects LIM Kinase from human samples.
PA1-31026 has been successfully used in Western blot applications.
The PA1-31026 immunogen is: Synthetic peptide: ETYRRGESGLPAHPEVPD, corresponding to amino acids 630-647 of Human LIM Kinase.
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: LIM domain kinase 1; LIM motif-containing protein kinase; LIMK; LIMK-1
Gene Aliases: LIMK; LIMK-1; LIMK1
UniProt ID: (Human) P53667
Entrez Gene ID: (Human) 3984
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