|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
|Tested Species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Bacterially expressed recombinant fragment (amino acids 86-202) of mouse LIME with C-terminal His-tag|
|Storage buffer||PBS, pH 7.4|
|Contains||15mM sodium azide|
|Storage conditions||4° C, do not freeze|
This antibody reacts with the cytoplasmic domain of LIME, and will not cross-react with human.
LIME (Lck-interacting molecule) is a 30 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Lck-interacting molecule; Lck-interacting transmembrane adapter 1; Lime1
Gene Aliases: 2810038K19Rik; LIME; Lime1
UniProt ID: (Mouse) Q9EQR5
Entrez Gene ID: (Mouse) 72699
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