|ChIP assay (ChIP)||1-3 ul|
|Flow Cytometry (Flow)||1:100|
|Immunofluorescence (IF)||1:50 - 1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:200|
|Immunoprecipitation (IP)||5 µg|
|Western Blot (WB)||1:1000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Full-length human recombinant protein expressed in bacteria|
|Storage buffer||PBS with 30% glycerol, 1mg/ml BSA|
|Contains||0.05% sodium azide|
MA1-016 has been successfully used in Western blot, immunofluorescence, ChIP, flow cytometry, IHC (P) and immunoprecipitation applications on human and mouse samples.
LIN28A/B are expressed in various cancers and ES cells. LIN28 is a cytoplasmic protein, that acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. LIN28 is a negative regulator of microRNA (miRNA) biogenesis by specifically binding the precursor of let-7 (pre-let-7). Let-7 miRNAs have been implicated in repression of oncogenes such as ras and myc. LIN28 expression decreases during differentiation of ES cells or upon induction of neuronal differentiation by retinoic acid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CSDD1; Lin-28 homolog A (C. elegans); Lin-28A; LIN28A; Protein lin-28 homolog A; RNA-binding protein LIN-28; RNA-binding protein LIN-28; testis expressed gene 17; Testis-expressed protein 17; ZCCHC1; Zinc finger CCHC domain-containing protein 1; zinc finger, CCHC domain containing 1
Gene Aliases: AL024421; CSDD1; ENSMUSG00000070700; Gm10299; LIN-28; lin-28A; LIN28; LIN28A; Tex17; ZCCHC1
Molecular Function: DNA binding protein
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