|Flow Cytometry (Flow)||1:25|
|Western Blot (WB)||1:1000|
|Tested Species reactivity||Human, Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to carboxy-terminal residues of human LIMK2|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
|Contains||<0.02% sodium azide|
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with LIMK1.
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: LIM domain kinase 2; LIM kinase 2; LIMK-2; LINhp-2; OTTHUMP00000199483
Gene Aliases: Limk-2; LIMK2
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