|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Synthetic peptide corresponding to human LMO2 protein|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.
LMO2 encodes a cysteine-rich, two LIM-domain protein that is required for yolk sac erythropoiesis. The LMO2 protein has a central and crucial role in hematopoietic development and is highly conserved. The LMO2 transcription start site is located approximately 25 kb downstream from the 11p13 T-cell translocation cluster (11p13 ttc), where a number T-cell acute lymphoblastic leukemia-specific translocations occur. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cysteine-rich protein TTG-2; LIM domain only 2 (rhombotin-like 1); LIM domain only protein 2; LMO-2; RBTN2; RBTNL1; RHOM2; rhombotin 2; Rhombotin-2; rhombotin-like 1; T-cell translocation gene 2; T-cell translocation protein 2; TTG2
Gene Aliases: LMO2; RBTN2; RBTNL1; RHOM2; TTG2
UniProt ID: (Human) P25791
Entrez Gene ID: (Human) 4005
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