|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:250|
|Western Blot (WB)||1.0-2 µg/ml|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic internal peptide corresponding to an internal region (within residues 300-400) of human LPIN1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
Suggested positive control: 3T3-L1 cell lysates, antigen standard for LPIN1 (transient overexpression lysate).
The Lipin family of nuclear proteins contains three members: Lipin-1, Lipin-2 and Lipin-3, all of which contain a nuclear signal sequence, a highly conserved amino-terminal (NLIP) domain and a carboxy-terminal (CLIP) domain. LPIN1 (Lipin-1) is crucial for normal adipose tissue development and metabolism. LPIN1 selectively activates a subset of PGC-1á target pathways, including fatty acid oxidation and mitochondrial oxidative phosphorylation by inducing expression of the nuclear receptor PPARá. LPIN1 also inactivates the lipogenic program and suppresses circulating lipid levels. An abundance of LPIN1 promotes fat accumulation and insulin sensitivity, whereas a deficiency in LPIN1 may deter normal adipose tissue development, resulting in insulin resistance and lipodystrophy, a heterogeneous group of disorders characterized by loss of body fat, fatty liver, hypertriglyceridemia and insulin resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DKFZp781P1796; Fatty liver dystrophy protein; KIAA0188; lipin 1; Lipin-1; LPIN1; Phosphatidate phosphatase LPIN1
Gene Aliases: 4631420P06; Fld; KIAA0188; Lipin1; LPIN1; mKIAA0188; PAP1
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