|Immunohistochemistry (Frozen) (IHC (F))||Assay-Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Western Blot (WB)||1:1000-1:2500|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an C-terminal portion of the human LDHA protein (within residues 280-332).|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 30% glycerol|
|Contains||0.1% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
This antibody is predicted to react with bovine and porcine based on 100% sequence homology.
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in a wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), due to the fact that it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C, forming various terameric isoenzymes (140 kDa). Lactate dehydrogenase A (LDH-A, muscle subunit, LDH-M) is involved in the final step of anaerobic glycolysis and catalyzes the conversion of L-lactate and NAD to pryruvate and NADH. While it is predominantly expressed in muscle tissue, it is hormonally regulated in rodents and overexpressed during mammary gland tumorigenesis (2). A mutation that causes deficiency in LDH-A has been implicated in exertional myoglobinuria (3).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Cell proliferation-inducing gene 19 protein; epididymis secretory sperm binding protein Li 133P; GSD11; L-lactate dehydrogenase A chain; lactate dehydrogenase 1, A chain; lactate dehydrogenase A4; lactate dehydrogenase M; lactate dehydrogenase-A; LDH muscle subunit; LDH-A; LDH-M; LDH1; LDHM; M-LDH; PIG19; proliferation-inducing gene 19; Renal carcinoma antigen NY-REN-59
Gene Aliases: GSD11; HEL-S-133P; l7R2; Ldh-1; Ldh1; LDHA; LDHM; PIG19
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.