|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Tested Species reactivity||Dog, Human|
|Host / Isotype||Sheep / IgG|
|Immunogen||Lactoferrin purified from milk.|
|Storage buffer||PBS, pH 7.4|
|Storage conditions||4°C or -20°C if preferred|
Heat-mediated antigen retrieval using a sodium citrate buffer (pH 6.0) is recommended for the staining of paraffin sections. A suggested positive control for immunohistochemical applications is mammary gland.
Lactoferrin (LTF) is an 80kDa non-haem, iron-binding globular glycoprotein and member of the transferrin family present in milk and mucosal secretions, and also released from neutrophil granules during inflammation. LTF acts as a major first line defense against microbial infections, partly due to its ability to efficiently bind to and hence remove Fe3+ from the environment, but studies have also shown that LTF possesses bactericidal properties and proteolytic activity, capable of cleaving arginine-rich sequences within microbial proteins. Three isoforms of LTF have been identified: LTF-alpha, LTF-beta and LTF-gamma and receptors for LTF are expressed in the gastrointestinal tract, by macrophages, lymphocytes, neutrophils and platelets and also by some bacteria.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: epididymis luminal protein 110; GIG12; Growth-inhibiting protein 12; HLF2; kaliocin-1; lactoferricin; Lactoferrin; lactoferroxin; Lactotransferrin; LF; neutrophil lactoferrin; Talalactoferrin
Gene Aliases: GIG12; HEL110; HLF2; LF; LTF
UniProt ID: (Human) P02788
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