|Immunocytochemistry (ICC)||2-5 µg/ml|
|Immunofluorescence (IF)||2-5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1, Kappa|
|Immunogen||Recombinant human livin protein.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: U266, HeLa, Jurkat.
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, Kasof and Gomes (1), Vucic et al. (2) and Ashhab et al. (3) have identified a novel human inhibitor of apoptosis protein (IAP) family member termed Livin or ML-IAP, which contains a single baculoviral IAP repeat (BIR) domain and a COOH-terminal RING finger domain. Livin gene has two splicing variants that contain open reading frames of 298 and 280 amino acids and both contained a single copy of baculovirus IAP repeat (BIR) and RING domain. Both of the isoforms inhibit TNFa induced apoptosis in Jurkat cells. Livin expression is low in adult tissues. It is relatively expressed at a higher level in developmental tissues and in many cancer cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Baculoviral IAP repeat-containing protein 7; KIAP; Kidney inhibitor of apoptosis protein; LIVIN; livin inhibitor of apoptosis; Melanoma inhibitor of apoptosis protein; ML-IAP; MLIAP; RING finger protein 50; RING-type E3 ubiquitin transferase BIRC7; RNF50; RP11-261N11.7
Gene Aliases: BIRC7; KIAP; LIVIN; ML-IAP; MLIAP; RNF50; UNQ5800/PRO19607/PRO21344
UniProt ID: (Human) Q96CA5
Entrez Gene ID: (Human) 79444
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