|Immunofluorescence (IF)||10-30 ug/ml|
|Immunohistochemistry (Frozen) (IHC (F))||15-50 ug/ml|
|Western Blot (WB)||1-3 ug/ml|
|Miscellaneous PubMed (MISC)||See 2 publications below|
|Immunofluorescence (IF)||See 1 publications below|
|Tested Species reactivity||Luciferase|
|Published species reactivity||Rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Luciferase protein from Photinus pyralis|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
Reactivity was confirmed via Western Blot analysis on the following samples containing added purified luciferase protein: mouse heart, liver, kidney, and cortex tissues. There was no cross reactivity to mouse heart, NIH3T3, Jurkat, and human fibroblasts. In IHC (frozen tissue) and Immunofluorescence applications, this antibody is able to detect tissue specific expression of Luciferase protein in Drosophila embryos using the well-characterized Gal4-UAS system with two different Gal4 drivers.
Luciferase is a luminescent enzyme produced by Photinus pyralis, also referred to as the common eastern firefly or North American firefly. It is known for its role as a reporter protein and can be used to quantify expression levels from promoters in tissue culture and in vivo. Luciferase catalyses the oxygenation of luciferin using ATP and oxygen to produce oxyluciferin, a highly unstable, singlet-excited compound that emits light upon relaxation to its ground state. Bioluminescence assay systems have become increasingly used in biology and medical research laboratories in addition to (or as alternatives to) fluorescence and chemiluminescence detection strategies. Luciferase enzymes isolated from different animal species have inherent variability in light emission, allowing two or more luciferase enzymes to be used in combination for multiplex analyses, including in vivo imaging, cell viability and single and dual-spectral luciferase reporter assays.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Firefly Luciferase; Genji firefly; Green Luciferase; Japanese firefly; Luciferin 4-monooxygenase; Photinus pyralis
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