|Western Blot (WB)||1:200-1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Sheep|
|Immunogen||A synthetic peptide from human MAP1LC3C conjugated to an immunogenic carrier protein|
|Storage buffer||whole serum|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Reconstitute with 150 ul of distilled water.
Centrifuge to remove any insoluble material.
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. Probably involved in formation of autophagosomal vacuoles (autophagosomes). 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins. Most abundant in placenta, lung and ovary. The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Autoph; Autophagy-related protein LC3 C; Autophagy-related ubiquitin-like modifier LC3 C; LC3-like protein 2; MAP1 light chain 3-like protein 2; MAP1 light chain 3-like protein 3; MAP1A/1B light chain 3 C; MAP1A/MAP1B LC3 C; MAP1A/MAP1B light chain 3 C; MAP1LC3C; Microtubule-associated protein 1 light chain 3 gamma; Microtubule-associated proteins 1A/1B light chain 3C
Gene Aliases: ATG8J; LC3C; MAP1LC3C
UniProt ID: (Human) Q9BXW4
Entrez Gene ID: (Human) 440738
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