|Western Blot (WB)||1:1000|
|Tested Species reactivity||Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues surrounding Cys140 of mouse MAVS/VISA|
|Storage buffer||0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/ml BSA, 50% glycerol|
It is not recommended to aliquot this antibody.
Double-stranded RNA viruses are recognized in a cell type-dependent manner by the transmembrane receptor TLR3 or by the cytoplasmic RNA helicases MDA5 and RIGI (ROBO3; MIM 608630). These interactions initiate signaling pathways that differ in their initial steps but converge in the activation of the protein kinases IKKA (CHUK; MIM 600664) and IKKB (IKBKB; MIM 603258), which activate NFKB, or TBK1 and IKKE (IKBKE; MIM 605048), which activate IRF3. Activated IRF3 and NFKB induce transcription of IFNB (IFNB1; MIM 147640). For the TLR3 pathway, the intermediary molecule before the pathways converge is the cytoplasmic protein TRIF (TICAM1; MIM 607601). For RIGI, the intermediary protein is mitochondria-bound IPS1 (Sen and Sarkar, 2005).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CARD adapter inducing interferon beta; CARD adapter inducing interferon-beta; CARD adaptor inducing IFN-beta; Cardif; IFN-B promoter stimulator 1; IFN-beta promoter stimulator-1; Interferon beta promoter stimulator protein 1; interferon-beta promoter stimulator protein 1; IPS-1; MAVS; mitochondrial anti-viral signaling protein; Mitochondrial antiviral-signaling protein; putative NF-kappa-B-activating protein 031N; virus-induced signaling adapter; virus-induced signaling adapter variant 1b; virus-induced signaling adaptor; virus-induced signaling adaptor variant 1a; Virus-induced-signaling adapter; VISA
Gene Aliases: cardif; D430028G21Rik; IPS-1; Ips1; Mavs; Visa
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