|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 123 and 349 of Human MBD2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-28277 targets MBD2 in WB applications and shows reactivity with Human samples.
The PA5-28277 immunogen is recombinant fragment corresponding to a region within amino acids 123 and 349 of Human MBD2.
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. The protein encoded by this gene may function as a mediator of the biological consequences of the methylation signal. It is also reported that the this protein functions as a demethylase to activate transcription, as DNA methylation causes gene silencing.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Demethylase; DMTase; Methyl-CpG-binding domain protein 2; Methyl-CpG-binding protein MBD2; NY-CO-41
Gene Aliases: DMTase; MBD2; NY-CO-41
UniProt ID: (Human) Q9UBB5
Entrez Gene ID: (Human) 8932
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