|Flow Cytometry (Flow)||1/200 - 1/400|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Immunohistochemistry (IHC)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human MCM2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA5-15923 targets MCM2 in FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15923 immunogen is purified recombinant fragment of human MCM2 expressed in E. Coli. .
MA5-15923 detects MCM2 which has a predicted molecular weight of approximately 125kDa.
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BM28, CCNL1, CDCL1, D3S3194, MITOTIN, cdc19, DNA replication licensing factor MCM2; cell devision cycle-like 1; cyclin-like 1; DNA replication licensing factor MCM2; MCM2 minichromosome maintenance deficient 2, mitotin; minichromosome maintenance deficient 2 (mitotin); Minichromosome maintenance protein 2 homolog; Nuclear protein BM28
Gene Aliases: BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; KIAA0030; MCM2; MITOTIN
UniProt ID: (Human) P49736
Entrez Gene ID: (Human) 4171
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