|Immunohistochemistry (Paraffin) (IHC (P))||0.5-1.0 µg/ml|
|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 6 publications below|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Dog, Human, Mouse, Rat, Xenopus|
|Published species reactivity||Dog , Rabbit , Mouse Human|
|Host / Isotype||Mouse / IgG1|
|Clone||47DC141 or DCS-141|
|Immunogen||Recombinant hCDC47 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14291 targets CDC47 in IF, IHC (P), IP, and WB applications and shows reactivity with Canine, Human, mouse, Rat, and Xenopus laevis samples.
The MA5-14291 immunogen is recombinant hCDC47 protein.
hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47-positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CDC47 homolog; CDC47 homolog A; CDC47p; DNA replication licensing factor MCM7; DNA replication licensing factor mcm7-A; homolog of S. cerevisiae Cdc47; mini chromosome maintenance deficient 7; minichromosome maintenance deficient 7; minichromosome maintenance protein 7; Minichromosome maintenance protein 7-A; P1.1-MCM3; p90; protein phosphatase 1, regulatory subunit 104; XMCM7; xMCM7-A
Gene Aliases: AI747533; CDC47; mCDC47; MCM2; MCM7; mcm7-a; Mcmd7; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.