|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 642 and 954 of Human MDC1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 1% BSA, 20% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27757 targets MDC1 in IHC (P) and WB applications and shows reactivity with Human samples.
The PA5-27757 immunogen is recombinant fragment corresponding to a region within amino acids 642 and 954 of Human MDC1.
The protein encoded by this gene contains an N-terminal forkhead domain, two BRCA1 C-terminal (BRCT) motifs and a central domain with 13 repetitions of an approximately 41-amino acid sequence. The encoded protein is required to activate the intra-S phase and G2/M phase cell cycle checkpoints in response to DNA damage. This nuclear protein interacts with phosphorylated histone H2AX near sites of DNA double-strand breaks through its BRCT motifs, and facilitates recruitment of the ATM kinase and meiotic recombination 11 protein complex to DNA damage foci.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: homologue to Drosophila photoreceptor protein calphotin; mediator of DNA damage checkpoint 1; Mediator of DNA damage checkpoint protein 1; mediator of DNA-damage checkpoint 1; NFBD1; Nuclear factor with BRCT domains 1
Gene Aliases: KIAA0170; MDC1; NFBD1
UniProt ID: (Human) Q14676
Entrez Gene ID: (Human) 9656
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