|Immunocytochemistry (ICC)||1 ug/ml|
|Immunofluorescence (IF)||1 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||2.5 µg/ml|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1:50-1:500|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||Human MDM2 protein.|
|Storage buffer||0.05M sodium phosphate, pH 7.5, with 0.2% gelatin|
|Contains||0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
MA1-25896 detects MDM2 from human samples.
MA1-25896 has been successfully used in immunofluorescence, immunohistochemistry (paraffin), immunoprecipitation, and Western blot procedures. Heat pretreatment is required for paraffin sections.
The MA1-25896 immunogen is human MDM2 protein.
This gene is a target gene of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Overexpression of this gene can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle, apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5. More than 40 different alternatively spliced transcript variants have been isolated from both tumor and normal tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2
Gene Aliases: ACTFS; hdm2; HDMX; MDM2
UniProt ID: (Human) Q00987
Entrez Gene ID: (Human) 4193
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