|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:500|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:500|
|Immunoprecipitation (IP)||Assay dependent|
|Immunohistochemistry (IHC)||See 14 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 2 publications below|
|Western Blot (WB)||See 5 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Mouse , Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Synthetic peptide corresponding to amino acids 154-167 (CSRPSTSSRRRAISE) of human MDM2.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
MA1-23318 detects MDM2 in Human samples.
MA1-23318 has been successfully used in Immunohistochemistry (Frozen, Paraffin) and Immunoprecipitation procedures.
MA1-23318 immunogen corresponds to Synthetic peptide corresponding to amino acids 154-167 (CSRPSTSSRRRAISE) of human MDM2.
Clone SMP14 recognizes the 90kDa MDM2 protein. MDM2 forms a complex with p53 which leads to inhibition of p53 function. MDM2 has also been shown to stimulate cellular proliferation via induction of transcriptional regulators including E2F1 and DP1. MDM2 is over expressed in a wide range of human malignancies including soft tissue carcinomas and breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Double minute 2 protein; double minute 2, human homolog of; p53-binding protein; E3 ubiquitin-protein ligase Mdm2; Hdm2; HDMX; MDM2 oncogene, E3 ubiquitin protein ligase; MDM2 proto-oncogene, E3 ubiquitin protein ligase; Mdm2, p53 E3 ubiquitin protein ligase homolog; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; MGC5370; MGC71221; Oncoprotein Mdm2; p53-binding protein Mdm2; RING-type E3 ubiquitin transferase Mdm2
Gene Aliases: ACTFS; hdm2; HDMX; MDM2
UniProt ID: (Human) Q00987
Entrez Gene ID: (Human) 4193
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.