|Immunofluorescence (IF)||20 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||17 amino acid peptide near the center of human ME2|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
A recommended positive control is human spleen tissue lysate.
At least two isoforms of ME2 are known to exist; this antibody will detect both isoforms. ME2 antibody is not expected to cross-react with ME1.
ME2 is a homotetrameric, mitochondrial NAD-dependent malic enzyme that catalyzes the oxidative decarboxylation of malate to pyruvate. It is related to malic enzyme 1 (ME1), a cytoplasmic NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The expression of both malic enzymes is reciprocally regulated by p53; this regulation has been shown to modulate metabolism and senescence. Certain single-nucleotide polymorphism haplotypes of the ME2 gene have been shown to increase the risk for idiopathic generalized epilepsy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: malate dehydrogenase; malate dehydrogenase (oxaloacetate-decarboxylating); Malic enzyme 2; malic enzyme 2, NAD(+)-dependent, mitochondrial; mitochondrial; NAD-dependent malic enzyme, mitochondrial; NAD-ME; ODS1; pyruvic-malic carboxylase
Gene Aliases: ME2; ODS1
UniProt ID: (Human) P23368
Entrez Gene ID: (Human) 4200
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