|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
|Tested Species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 346 and 507 of Human MEF2A|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA5-27380 targets MEF2A in IF and WB applications and shows reactivity with Human samples.
The PA5-27380 immunogen is recombinant fragment corresponding to a region within amino acids 346 and 507 of Human MEF2A.
The process of differentiation from mesodermal precursor cells to myoblasts has led to the discovery of a variety of tissue-specific factors that regulate muscle gene expression. The myogenic basic helix-loop-helix proteins, including myoD (MIM 159970), myogenin (MIM 159980), MYF5 (MIM 159990), and MRF4 (MIM 159991) are one class of identified factors. A second family of DNA binding regulatory proteins is the myocyte-specific enhancer factor-2 (MEF2) family. Each of these proteins binds to the MEF2 target DNA sequence present in the regulatory regions of many, if not all, muscle-specific genes. The MEF2 genes are members of the MADS gene family (named for the yeast mating type-specific transcription factor MCM1, the plant homeotic genes 'agamous' and 'deficiens' and the human serum response factor SRF (MIM 600589)), a family that also includes several homeotic genes and other transcription factors, all of which share a conserved DNA-binding domain.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: ADCAD1; MADS box transcription enhancer factor 2; MADS box transcription enhancer factor 2, polypeptide A (myocyte enhancer factor 2A); mef2; Myocyte-specific enhancer factor 2A; polypeptide A (myocyte enhancer factor 2A); RSRFC4; RSRFC9; Serum response factor-like protein 1
Gene Aliases: A430079H05Rik; ADCAD1; MEF2; MEF2A; RSRFC4; RSRFC9
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