|Flow Cytometry (Flow)||1:10-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500-1:1000|
|Western Blot (WB)||1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 5 publications below|
|Immunohistochemistry (IHC)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Immunogen||Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: CEM-CCRF cells.
TK6 cells as a negative control.
O6-Methylguanine-DNA Methyltransferase (MGMT) is an important DNA repair protein involved in tumor cell resistance to the cytotoxic activity of chemotherapeutic alkylating agents. This protein is also effective in protecting normal cells against the genotoxic and carcinogenic effects of DNA alkylation. The alkylating drug resistance is caused by MGMT's ability to remove DNA alkyl groups introduced in the O6 position of guanine. MGMT is expressed in highly variable amounts, depending upon the cell and tissue type, species, and cellular growth characteristics. In addition, MGMT activity varies among groups of tumors and within a particular type of tumor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6-O-methylguanine-DNA methyltransferase; methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; METHYLGUANINE-DNA METHYLTRANSFERASE; MGMT; MGMT: O-6-methylguanine-DNA methyltransferase; O-6-methylguan; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase
Gene Aliases: MGMT
UniProt ID: (Human) P16455
Entrez Gene ID: (Human) 4255
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