|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10 - 1:20|
|Western Blot (WB)||0.5-1.0 µg/ml|
|Immunohistochemistry (IHC)||See 36 publications below|
|Western Blot (WB)||See 12 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|ELISA (ELISA)||See 1 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant human MGMT protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13503 targets MGMT (O-methylguanine-DNA methyltransferase) in FACS, IHC (P), ICC/IF and WB applications and shows reactivity with Human samples.
The MA5-13503 immunogen is purified recombinant human MGMT protein.
Cancer chemotherapeutic alkylating agents (e.g. BCNU,) act by inducing formation of lethal cross-links at the O-alkylguanine position in DNA. MGMT transfers alkyl adducts from the O-position of guanine in DNA (prior to cross-link formation) to a cysteine residue in its own sequence, thereby restoring DNA to its intact state. This transfer inactivates the MGMT enzyme and is irreversible; hence the level of MGMT in a cell is directly proportional to the level of DNA-damage it can tolerate. In normal tissues, MGMT acts as a suppressor of mutation and carcinogenesis. Tumors with high levels of MGMT are likely be drug-resistant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 6-O-methylguanine-DNA methyltransferase; methylated DNA protein cysteine methyltransferase; Methylated-DNA--protein-cysteine methyltransferase; methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase; O6-methylguanine-DNA methyltransferase
Gene Aliases: MGMT
UniProt ID: (Human) P16455
Entrez Gene ID: (Human) 4255
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.