|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1-2,000-1:10,000|
|Immunocytochemistry (ICC)||See 1 publications below|
|Immunofluorescence (IF)||See 2 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Human , Mouse Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant rat MIF fusion protein|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
36-7401 detects MIF from human, mouse and rat samples.
36-7401 has been successfully used in Western blot, immunohistochemical, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects a 12 kDa protein representing MIF. Immunofluorescent staining in U2OS cells and Immunohistochemical staining of MIF in rat epididymis tissue with 36-7401. yields a staining pattern consistent with MIF in nucleus and cytosol.
MIF (Macrophage Migration Inhibitory Factor) is a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through suppression of the anti-inflammatory effects of glucocorticoids. MIF may also play a role in integrin signaling pathways, because it has been found to form a complex in the cytosol near the peripheral plasma membrane with the JAB1 protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Delayed early response protein 6; DER6; GIF; GLIF; glutathione-binding 13 kDa protein; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; Macrophage migration inhibitory factor; MIF; MMIF; Phenylpyruvate tautomerase
Gene Aliases: GIF; GLIF; MIF; MMIF
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