|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to internal region of human MIP-1alpha|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is lung tissue.
Macrophage inflammatory protein-1 alpha (MIP-1 alpha) is an 8kDa member of the C-C chemokine subfamily. C-C chemokines are chemoattractants and activators for monocytes and T cells. MIP-1 alpha has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1b and RANTES.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-C motif chemokine 3; chemokine (C-C motif) ligand 3; G0/G1 switch regulatory protein 19-1; G0S19-1; LD78ALPHA; Macrophage inflammatory protein 1-alpha; MIP-1-alpha; MIP1A; PAT 464.1; SCYA3; SIS-beta; small inducible cytokine A3 (homologous to mouse Mip-1a); small inducible cytokine A3 homologous to mouse Mip-1a; Small-inducible cytokine A3; Tonsillar lymphocyte LD78 alpha protein
Gene Aliases: CCL3; G0S19-1; LD78ALPHA; MIP-1-alpha; MIP1A; SCYA3
UniProt ID: (Human) P10147
Entrez Gene ID: (Human) 6348
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