|Western Blot (WB)||1:2000|
|Tested Species reactivity||Human|
|Host / Isotype||Chicken / IgY|
|Immunogen||Synthetic peptide corresponding to human Microphthalmia-associated transcription factor.|
|Purification||Antigen affinity chromatography|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-27613 detects MITF from human samples.
PA1-27613 may be successfully used in Western blot applications. By Western blot, this antibody has only been tested on fusion protein.
The immunogen is a synthetic peptide corresponding to human Microphthalmia-associated transcription factor.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
Transcription factor for tyrosinase and tyrosinase-related protein 1. Binds to a symmetrical DNA sequence (E-boxes) (5'-CACGTG-3') found in the tyrosinase promoter. Plays a critical role in the differentiation of various cell types as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigmant epithelium. This protein is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrom type 2 and Tietz syndrome. Alternateively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHe32; Class E basic helix-loop-helix protein 32; MI; microphtalmia-associated transcription factor; Microphthalmia-associated transcription factor; WS2A
Gene Aliases: BHLHE32; CMM8; MI; MITF; WS2; WS2A
UniProt ID: (Human) O75030
Entrez Gene ID: (Human) 4286
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