|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||20-40 µg/mL|
|Western Blot (WB)||5-10 µg/mL|
|Western Blot (WB)||See 1 publications below|
|Immunohistochemistry (IHC)||See 6 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human , Chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide from the middle region of the human MMP-1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
PA5-16498 targets MMP-1 (Collagenase-1) in IHC (P) and WB applications and shows reactivity with Human samples. Antigen retrieval via boiling in 10 mM citrate buffer, pH 6.0 for 10 - 20 minutes followed by cooling to RT for 20 minutes is required for formalin fixed, paraffin embedded tissues.
The PA5-16498 immunogen is a synthetic peptide from the middle region of the human MMP-1.
Matrix metalloproteinases (MMP) are proteolytic enzymes capable of degrading connective tissue components. Imbalanced secretion of certain MMP or disturbances in the differential control of MMP by tissue inhibitor of MMPs (TIMPs) are implicated in the invasive potential of malignant tumors. Interstitial collagenase (MMP-1), 72kDa gelatinase A/type IV collagenase (MMP-2), and 92kDa gelatinase B/type IV collagenase (MMP-9) dissolve extracellular matrix (ECM) and may initiate and promote angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Fibroblast collagenase; Interstitial collagenase; matrix metallopeptidase 1 (interstitial collagenase); matrix metalloprotease 1; matrix metalloproteinase 1; Matrix metalloproteinase-1; MMP-1
Gene Aliases: CLG; CLGN; MMP1
UniProt ID: (Human) P03956
Entrez Gene ID: (Human) 4312
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