|Immunofluorescence (IF)||Assay Dependent|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 23 publications below|
|Western Blot (WB)||See 11 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Human native 72kDa MMP-2|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-14186 targets MMP-2 (72kDa Collagenase IV) in IF, IP, and WB applications and shows reactivity with Human samples.
The MA5-14186 immunogen is human native 72kDa MMP-2.
MMPs are proteolytic enzymes capable of degrading connective tissue components. They have a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and are inhibited by specific tissue inhibitors of metalloproteinases (TIMPs). MMPs and TIMPs play a significant role in regulating angiogenesis. MMP-2; also known as 72kDa collagenase IV or gelatinase A is synthesized as a 631 amino acid proenzyme which is activated by cleavage of the first 80 amino acids.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 72 kDa gelatinase; 72 kDa type IV collagenase; collagenase type IV-A; Gelatinase A; matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase); Matrix metalloproteinase-2; matrix metalloproteinase-II; MMP-2; neutrophil gelatinase; TBE-1
Gene Aliases: CLG4; CLG4A; MMP-2; MMP-II; MMP2; MONA; TBE-1
UniProt ID: (Human) P08253
Entrez Gene ID: (Human) 4313
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.