|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein encoding aa 144-451 of human MUM1 protein.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is Hodgkin's lymphoma.
The MUM1 (Multiple Myeloma Oncogene 1) gene was originally identified because of its involvement in the t(6;14) translocation observed in multiple myeloma, which causes juxtaposition of the MUM1 gene to the lg heavy-chain locus. MUM1 is expressed in a wide spectrum of lymphoid neoplasms but not in myeloid and extra-hematopoitic tumors. MUM1 is a valuable marker for understanding and characterizing histogenensis of B-cell lymphomas. It is an excellent marker for Hodgkin's and Reed-Sternberg cells of classic Hodgkin's disease in combination with CD30.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Interferon regulatory factor 4; IRF-4; LSIRF; Lymphocyte-specific interferon regulatory factor; Multiple myeloma oncogene 1; MUM1; NF-EM5
Gene Aliases: IRF4; LSIRF; MUM1; NF-EM5; SHEP8
UniProt ID: (Human) Q15306
Entrez Gene ID: (Human) 3662
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