|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:500|
|Immunoprecipitation (IP)||1 µg/ml|
|Western Blot (WB)||1 µg/ml|
|Western Blot (WB)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 1 publications below|
|Immunocytochemistry (ICC)||See 3 publications below|
|Tested Species reactivity||Cat, Human, Mouse, Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant mouse MyoD.|
|Contains||0.05% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.
Suggested positive control: Rh-30.
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: bHLHc1; Class C basic helix-loop-helix protein 1; Myf-3; MYF3; Myoblast determination protein 1; MYOD; Myogenic factor 3
Gene Aliases: AI503393; BHLHC1; MYF3; MYOD; Myod-1; MYOD1; PUM
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