|Flow Cytometry (Flow)||1/50 - 1/100|
|Immunohistochemistry (Frozen) (IHC (F))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
|Immunohistochemistry (Paraffin) (IHC (P))||See 1 publications below|
|Immunohistochemistry (IHC)||See 11 publications below|
|Tested Species reactivity||Bovine, Dog, Deer, Horse, Cat, Guinea pig, Goat, Human, Non-human primate, Pig, Rabbit, Rat|
|Published species reactivity||Dog , Pig , Bovine , Human , Mouse|
|Host / Isotype||Mouse / IgG1|
|Contains||0.09% sodium azide|
|Storage conditions||4°C or -20°C if preferred|
This clone recognizes the L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. Although originally described as binding to epitopes common to both the alpha and beta chains (Flavell et al. 1987) subsequent studies indicate that the antibody detects an epitope exclusively expressed on the beta chain (Goebeler et al. 1994) demonstrated by immunofluorescent and western blotting on both naturally expressing and transfected targets. In addition Mouse anti Human macrophages, clone MAC387 detects the beta chain in complex with the alpha. The antigen recognized by Mouse anti Human macrophages, clone MAC387 is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia. The epitope recognized appears to be well conserved and the antibody is routinely used for the detection of myeloid cellds in a wide range of species.
This product requires protein digestion pre-treatment of paraffin sections using trypsin or pronase prior to IHC staining. A suggested positive control is human spleen. For flow cytometry, use 10ul of the suggested working dilution to label 1x10^6 cells in 100ul.
L1, or Calprotectin molecule, is an intracytoplasmic antigen comprised of a 12 kDa alpha chain and a 14 kDa beta chain. The antigen is expressed by granulocytes, monoctyes and tissue macrophages.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 60B8AG; CAGB; CGLB; L1AG; LIAG; MAC387; MRP14; NIF
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