|Immunofluorescence (IF)||1:20 - 1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:20-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:50|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG2b|
|Storage buffer||ascites with 1% BSA|
|Contains||0.1% sodium azide|
|Storage conditions||Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C|
For improved staining on formalin-fixed sections, it is recommended to preincubate with 10mM citrate buffer, pH 6.0, for 5 mintues at 720 Watts in a microwave oven. Material fixed with sensofix and sublimate buffered formalin do not need pretreatment.
Natural killer (NK) cells in teleosts and their evolutionary homologue are a subpopulation of lymphocytes with properties that distinguish them from either B- or T-cells. NK cells are important effectors of innate immunity where they release cytokines, which in turn up-regulate other immunological functions. Monoclonal antibodies have been used to identify different surface antigens present on NK cells. These surface antigens have not only been used to identify NK cells, but also their functionally distinct subsets.
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