|Flow Cytometry (Flow)||Assay Dependent|
|Gel Shift (GS)||1mg/mL|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-100|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 1 publications below|
|Western Blot (WB)||See 1 publications below|
|Tested Species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13871 targets Major Vault Protein in FACS, GS, IHC (P), IP, and WB applications and shows reactivity with Human and Rat samples.
The MA5-13871 immunogen is proteins precipitated with AER317 from the phosphocellulose column flow through of extract of human breast cancer MCF-7 cells.
Major vault protein (MVP) is identical to lung-resistance related protein (LRP). Its shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to progesterone and glucocorticoid receptors are also able to co-immunoprecipitate the MVP. Reportedly, the lung-resistance related protein (LRP) is identical to human MVP. LRP is overexpressed in many neoplastic tissues and cell lines indicating a poor response to chemotherapy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Lung resistance-related protein; Major vault protein; MVP; testicular secretory protein Li 30
Gene Aliases: LRP; MVP; VAULT1
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