|Flow Cytometry (Flow)||10 µl for 10^6 cells in 100 µl|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay dependent|
|Western Blot (WB)||Assay dependent|
|Tested Species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified human mannose receptor|
|Contains||0.09% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
MA1-34288 detects CD206 from human samples.
MA1-34288 has been successfully used in immunocytochemistry, immunofluorescence, immunohistochemistry (frozen tissue), Western blot and FACS applications. Use 10 ul of the suggested working dilution to label 10^6 cells in 100ul.
The MA1-34288 immunogen is purified human mannose receptor.
Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognizes a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MRs are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells. The expression of MR on monocytes increases during culture and can be enhanced by cytokines such as IFN-gamma. Labeling of MR expressing monocytes/macrophages increases at 37¡ãC with prolonged incubation time probably due to internalization of the MR-antibody-complex. The antibody prevents binding of glycoproteins including t-PA to MR. Detection of the MR with anti-MR monoclonal antibody can substitute staining for mannose containing probes as labeled mannosylated BSA, a technique which is more cumbersome and less specific.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: C-type lectin domain family 13 member D; macrophage mannose receptor 1; macrophage mannose receptor 1-like protein 1; mannose receptor, C type 1-like 1
Gene Aliases: bA541I19.1; CD206; CLEC13D; CLEC13DL; MMR; MRC1L1; RP11-457D2.1
UniProt ID: (Human) P22897
Entrez Gene ID: (Human) 4360
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