|Immunoprecipitation (IP)||2 µg/mg protein lysate|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 3 publications below|
|Western Blot (WB)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human Mcl-1 protein corresponding to aa 1-327|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-13929 targets Mcl-1 in immunoprecipitation and Western blot applications and shows reactivity with Human samples.
The MA5-13929 immunogen is recombinant human Mcl-1 protein corresponding to aa 1-327.
Mcl-1 (Myeloid cell leukemia-1) is a member of the bcl-2 family. The carboxy terminal of Mcl-1 and bcl-2 share significant sequence homology. Expression of Mcl-1 is increased upon exposure of ML-1 cells to various types of DNA damaging agents (e.g. ionizing radiation, ultraviolet radiation, and alkylating drugs) along with increases in GADD45 and Bax and a decrease in bcl-2. Enhanced expression of Mcl-1, prominently associated with mitochondria, complements the continued expression of bcl-2 in ML-1 cells undergoing differentiation. Like bcl-2, Mcl-1 has the capacity to promote cell viability under conditions that otherwise cause apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Bcl-2-like protein 3; Bcl-2-related protein EAT/mcl1; Bcl2-L-3; Induced myeloid leukemia cell differentiation protein Mcl-1; isoform 1; mcl1/EAT; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1; myeloid cell leukemia sequence 1 (BCL2-related)
Gene Aliases: bcl2-L-3; BCL2L3; EAT; Mcl-1; MCL1; MCL1-ES; mcl1/EAT; MCL1L; MCL1S; TM
UniProt ID: (Human) Q07820
Entrez Gene ID: (Human) 4170
Molecular Function: signaling molecule
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