|Flow Cytometry (Flow)||1:100|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100|
|Tested Species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to C-terminus of human mesothelin protein.|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage conditions||4° C, do not freeze|
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is mesothelioma.
Mesothelin is a glycocylphosphotidylianositol-linked cell-surface glycoprotein that is present on the surface of normal mesothelium and is overexpressed in epithelial ovarian cancer and malignant mesotheliomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CAK1 antigen; megakaryocyte potentiating factor; Mesothelin; MPF; Pre-pro-megakaryocyte-potentiating factor; soluble MPF mesothelin related protein
Gene Aliases: MPF; MSLN; SMRP
UniProt ID: (Human) Q13421
Entrez Gene ID: (Human) 10232
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