|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Western Blot (WB)||0.1-1 µg/ml|
|Immunohistochemistry (IHC)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: C P(469) N R E E P V D S R T P V T E R V S(486)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-888 detects methyl CpG binding protein 2 (MeCP2) from human, mouse and rat cells and tissues.
PA1-888 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~56 kDa protein representing MeCP2 from AtT20 cell extract.
PA1-888 immunizing peptide corresponds to amino acid residues 469-486 from human MeCP2. This sequence is completely conserved in rat and mouse MeCP2. PA1-888 immunizing peptide (Cat. # PEP-121) is available for use in neutralization and control experiments.
DNA methyltransferases methylate the 5-position of cytosine in the context of CpG dinucleotides. DNA methylation is crucial for normal embryonic development, imprinting, and X-chromosome inactivation. Methyl CpG binding proteins (MeCPs) specifically recognize methylated regions of DNA and repress transcription both directly and by association with known corepressor proteins which include members of the Sin3 and histone deactylase protein families.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: MeCp-2 protein; MeCP2; methyl CpG binding protein 2; Methyl-CpG-binding protein 2; testis tissue sperm-binding protein Li 41a
Gene Aliases: 1500041B07Rik; AUTSX3; D630021H01Rik; Mbd5; MECP2; MRX16; MRX79; MRXS13; MRXSL; PPMX; RS; RTS; RTT; WBP10
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.