|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic non-phosphopeptide derived from human MITF around the phosphorylation site of Ser180/73 (P-N-SP-P-M)|
|Purification||Antigen affinity chromatography|
|Storage buffer||Dulbecco's PBS, pH 7.4, with 150mM NaCl, 50% glycerol|
|Contains||0.02% sodium azide|
MITF is a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. Plays a critical role in the differentiation of various cell types including neural crest- derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. Two isoforms are known: the M-isoform is expressed exclusively in melanocytes, while the A-isoform has a much broader range of expression. Mutations in MITF can lead to Waardenburg syndrome. Ten alternatively spliced isoforms have been described.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: BHLHE32; black eyed white; Class E basic helix-loop-helix protein 32; microphtalmia-associated transcription factor; Microphthalmia-associated transcription factor; MITF
Gene Aliases: BCC2; BHLHE32; Bw; CMM8; Gsfbcc2; MI; MITF; Vit; Vitiligo; Wh; WS2; WS2A
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