|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
|Immunohistochemistry (IHC)||See 6 publications below|
|Western Blot (WB)||See 3 publications below|
|Immunocytochemistry (ICC)||See 2 publications below|
|Tested Species reactivity||Human|
|Published species reactivity||Rat Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Semi-purified mitochondrial preparation|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
This antibody recognizes a 60kDa non-glycosylated protein component of mitochondria found in normal and malignant human cells.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min followed by cooling at room temperature for 20 min. Recommended positive controls: HeLa cells and liver tissue.
Antibodies that recognize subcellular organelles or compartments of human cells are useful in identification of these organelles in cells, tissues, and biochemical preparations. Anti-mitochondria antibodies can be used to stain mitochondria in cell/tissue preparations and can be used as a marker in subcellular fractions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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