|Dot blot (DB)||1:5000-1:100,000|
|Western Blot (WB)||1:5000-1:100,000|
|Tested Species reactivity||Mouse|
|Host / Isotype||Goat / IgG|
|Immunogen||Mouse IgM mu chain|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10mg/ml BSA|
|Contains||0.01% gentamicin sulfate|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
PA1-29581 detects Mouse IgM, F(ab)2 from mouse samples.
PA1-29581 has been successfully used in ELISA, dot blot, and Western blot applications.
The PA1-29581 immunogen is Mouse IgM mu chain.
store at 4°C short term. For extended storage add 50% glycerol, aliquot and store at -20°C or below. Avoid freeze/thaw cycles.
Thermo Scientific Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.