|Flow Cytometry (Flow)||1-2 ug/test|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||1:10,000|
|Immunohistochemistry (Paraffin) (IHC (P))||See 3 publications below|
|Immunocytochemistry (ICC)||See 6 publications below|
|ChIP assay (ChIP)||See 4 publications below|
|Immunohistochemistry (IHC)||See 9 publications below|
|Western Blot (WB)||See 12 publications below|
|Immunoprecipitation (IP)||See 4 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 1 publications below|
|Tested Species reactivity||Human, Mouse|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Armenian hamster / IgG|
|Immunogen||A synthetic peptide corresponding to aa 239-255 (SSLSYTNPAVAATSANL) from the cytoplasmic tail of MUC1|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage conditions||4° C|
MA5-11202 detects smaller subunits (14-28kDa) of MUC-1.
Muc-1 is overexpressed in Breast cancer cells.
T47D cell lysate could be used as a positive control.
Muc1 is a heavily O-glycosylated transmembrane protein expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and overexpressed in >90% breast carcinomas and metastases. In normal mammary gland it is expressed in apical surface of glandular epithelium. In breast cancer Muc1 is overexpressed; is underglycosylated and the apical localization is lost. Muc1 is transcribed as a larger precursor which is cleaved to form a larger mucin like subunit (265-400kDa) and a smaller subunit (14-28kDa) noncovalently associated with each other. Transgenic Muc1 has been shown to associate with all four erbB receptors and localize with erbB1 (EGFR) in lactating glands1. Muc1 can act as a ligand for ICAM-1 on HUVEC cells; it can bind beta-catenin, GSK3beta and it associates with Grb2-SOS upon phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Breast carcinoma-associated antigen DF3; CA 15-3; Cancer antigen 15-3; Carcinoma-associated mucin; CD227; DF3 antigen; EMA; Episialin; Epithelial Membrane Antigen; H23 antigen; H23AG; KL-6; Krebs von den Lungen-6; MAM6; Medullary cystic kidney disease, autosomal dominant; MUC-1; MUC-1/SEC; MUC-1/X; MUC1/ZD; mucin 1, transmembrane; Mucin-1; Peanut-reactive urinary mucin; PEM; PEMT; Polymorphic epithelial mucin; PUM; tumor associated epithelial mucin; Tumor-associated epithelial membrane antigen; Tumor-associated mucin
Gene Aliases: ADMCKD; ADMCKD1; CA 15-3; CD227; EMA; H23AG; KL-6; MAM6; MCD; MCKD; MCKD1; MUC-1; MUC-1/SEC; MUC-1/X; MUC1; MUC1/ZD; PEM; PEMT; PUM
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