|Flow Cytometry (Flow)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1,000|
|Western Blot (WB)||See 3 publications below|
|Tested Species reactivity||Human, Mouse, Pig, Rat|
|Published species reactivity||Rat , Pig Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified porcine cardiac m2 muscarinic acetylcholine receptor.|
|Storage buffer||1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA3-044 detects muscarinic 2 acetylcholine receptor (m2AChR) from human, mouse, pig and rat tissues. This antibody does not detect mAChR from chicken tissues. MA3-044 is specific for the m2 mAChR subtype.
MA3-044 has been successfully used in Western blot and immunoprecipitation protocols. By Western blot, this antibody detects 60-80 kDa protein(s) (depending on the degree of glycosylation) representing the m2AChR from pig heart.
The MA3-044 antigen is purified porcine cardiac m2 AChR.
Acetylcholine receptors (AChRs) mediate synaptic transmission at the neuromuscular junction. The channel-linked AChR that mediates rapid, excitatory actions of acetylcholine is called nicotinic AChR (nAChR) because it can be activated by nicotine. The non-channel linked AChR that medicates the slow actions of acetylcholine, which can be either inhibitory or excitatory, is called muscarinic AChR (mAChR) because it can be activated by muscarine.
The mAChRs are present in neurons of the central and peripheral nervous systems, cardiac and smooth muscle and various exocrine glands. There are 5 subtypes (m1-m5) of the receptor that have a tissue specific pattern of expression. The m2 receptor is localized primarily in cardiac tissue and is also expressed at low levels in the hippocampus, cortex, striatum, thalamus, basal forebrain, brainstem, lung, vas deferens and uterus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 7TM receptor; acetylcholine receptor, muscarinic 2; AChR M2; cardiac muscarinic acetylcholine receptor; cholinergic receptor, muscarinic 2; cholinergic receptor, muscarinic 2, cardiac; cholinergic receptor, muscarinic 2, isoform a; M2 muscarinic acetylcholine receptor; m2AChR; muscarinic acetylcholine receptor 2; Muscarinic acetylcholine receptor M2; muscarinic M2 receptor; muscarinic receptor m2
Gene Aliases: AChR-M2; Acm2; Chrm-2; CHRM2; HM2; M2
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