|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Western Blot (WB)||3-6 µg/ml|
|Immunocytochemistry (ICC)||See 2 publications below|
|Western Blot (WB)||See 2 publications below|
|Immunohistochemistry (IHC)||See 6 publications below|
|Tested Species reactivity||Rat, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic Peptide: S(16) G Q E F D V P I G A V V K L C(31)|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-936 detects myosin VIIa from mouse tissues as well as recombinant protein.
PA1-936 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~220 kDa protein representing myosin VIIa from mouse testes preparations. PA1-936 detects recombinant mouse myosin VIIa overexpressed in Sf9 insect cell lysate.
PA1-936 immunizing peptide corresponds to amino acid residues 16-31 from mouse myosin VIIa. This sequence is completely conserved in mouse, porcine, and human myosin VIIa. PA1-936 immunizing peptide (Cat. # PEP-045) is available for use in neutralization and control experiments.
Myosin VIIa is a member of the myosin superfamily of actin-based motor proteins. Defects in the myosin VIIa gene are responsible for hearing impairment in shaker-1 (sh1) mice and causes Usher syndrome IB in humans. Usher syndrome associates congenital deafness, vestibular dysfunction, and retinitis pigmentosa and is the most common form of combined deafness and blindness. Structural features of myosin VIIa protein include an ATP binding N-terminal motor domain, a central region which possess five light-chain binding (IQ) motifs, and a C-terminal domain with three myosin tail homology (MyTH4) and talin-like homology regions.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: motor protein; MyO VIIa; myosin VIIA (Usher syndrome 1B (autosomal recessive, severe)); myosin-VIIa; shaker 1; Unconventional myosin-VIIa
Gene Aliases: Hdb; Myo7; Myo7a; nmf371; polka; sh-1; sh1; USH1B
UniProt ID: (Mouse) P97479
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