|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||1 µg/ml|
|Immunohistochemistry (IHC)||See 18 publications below|
|Western Blot (WB)||See 36 publications below|
|Immunohistochemistry (Frozen) (IHC (F))||See 8 publications below|
|Immunohistochemistry (Paraffin) (IHC (P))||See 17 publications below|
|Immunocytochemistry (ICC)||See 9 publications below|
|Miscellaneous PubMed (MISC)||See 24 publications below|
|Immunofluorescence (IF)||See 4 publications below|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||See 2 publications below|
|Immunoprecipitation (IP)||See 2 publications below|
|Tested Species reactivity||Chicken, Human, Mouse, Pig, Rat|
|Published species reactivity||Tag , Rabbit , Rat , Non-human primate , Hamster , Mouse , Human , Horse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant domain corresponding to the intracellular domain of chicken N-Cadherin|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
33-3900 has successfully been used in western blot to detect N-cadherin in MDCK and mouse heart lysates.
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin's function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: cadherin 2 type 1 N-cadherin (neuronal); cadherin 2, type 1, N-cadherin (neuronal); Cadherin-2; calcium-dependent adhesion protein, neuronal; CD325; CDHN; CDw325; CDw325 antigen; N-cadherin; N-cadherin 1; NCAD; Neural cadherin
Gene Aliases: CD325; CDH2; CDHN; CDw325; N-cadherin; NCAD
Molecular Function: cadherin
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